Alternative Carcinogenicity Screening Assay Using Colon Cancer Stem Cells: A Quantitative PCR (qPCR)-Based Prediction System for Colon Carcinogenesis
Journal of Microbiology and Biotechnology 2018³â 28±Ç 4È£ p.645 ~ p.651
(Bak Ye-Sol) - Konkuk University Department of Bioscience and Biotechnology
(Jang Hui-Joo) - Konkuk University Department of Bioscience and Biotechnology
(Shin Jong-Woon) - Konkuk University Department of Bioscience and Biotechnology
±è¼öÁø(Kim Soo-Jin) - Konkuk University Department of Bioscience and Biotechnology
(Chun Hyun-Woo) - Konkuk University Department of Bioscience and Biotechnology
¼ÁöÇý(Seo Ji-Hye) - Jeonbuk National University School of Dentistry Department of Dental Pharmacology
(No Su-Hyun) - Jeonbuk National University School of Dentistry Department of Dental Pharmacology
äÁ¤ÀÏ(Chae Jung-Il) - Jeonbuk National University School of Dentistry Department of Dental Pharmacology
¼Õµ¿Èñ(Son Dong-Hee) - Sejong University College of Natural Sciences Department of Applied Statistics
À̽¿¬(Lee Seung-Yeoun) - Sejong University College of Natural Sciences Department of Applied Statistics
È«ÁøÅÂ(Hong Jin-Tae) - Chungbuk National University College of Pharmacy
À±µµ¿µ(Yoon Do-Young) - Konkuk University Department of Bioscience and Biotechnology
Abstract
The carcinogenicity of chemicals in the environment is a major concern. Recently, numerous studies have attempted to develop methods for predicting carcinogenicity, including rodent and cell-based approaches. However, rodent carcinogenicity tests for evaluating the carcinogenic potential of a chemical to humans are time-consuming and costly. This study focused on the development of an alternative method for predicting carcinogenicity using quantitative PCR (qPCR) and colon cancer stem cells. A toxicogenomic method, mRNA profiling, is useful for predicting carcinogenicity. Using microarray analysis, we optimized 16 predictive gene sets from five carcinogens (azoxymethane, 3,2¡¯-dimethyl-4-aminobiphenyl, N-ethyl-n-nitrosourea, metronidazole, 4-(n-methyl-n-nitrosamino)-1-(3-pyridyl)-1-butanone) used to treat colon cancer stem cell samples. The 16 genes were evaluated by qPCR using 23 positive and negative carcinogens in colon cancer stem cells. Among them, six genes could differentiate between positive and negative carcinogens with a p-value of ¡Â0.05. Our qPCR-based prediction system for colon carcinogenesis using colon cancer stem cells is cost- and time-efficient. Thus, this qPCR-based prediction system is an alternative to in vivo carcinogenicity screening assays.
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Microarray, quantitative PCR, colon cancer, cancer stem cell, carcinogenicity
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This qPCR-based prediction system is an alternative to in vivo carcinogenicity screening assays.